ARFIP2 Polyklonaler Antikörper

ARFIP2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14548-1-AP

Synonyme

Arfaptin 2, ARFIP2, Partner of RAC1, POR1, Protein POR1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14548-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14548-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14548-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14548-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 14548-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14548-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 14548-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 14548-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 14548-1-AP

  Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 14548-1-AP

  Immunofluorescent analysis of HepG2 cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14548-1-AP bindet in WB, IHC, IF, ELISA ARFIP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARFIP2 fusion protein Ag6076
• Vollständiger Name: ADP-ribosylation factor interacting protein 2
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa, 45 kDa
• GenBank-Zugangsnummer: BC000392
• Gene symbol: ARFIP2
• Gene ID (NCBI): 23647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	IHC,WB,IF	J Hepatocell Carcinoma; ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway; Authors - Kaida Huang