ARF5-Specific Polyklonaler Antikörper

ARF5-Specific Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20227-1-AP

Synonyme

ADP ribosylation factor 5, ARF5

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 20227-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20227-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20227-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20227-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 20227-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20227-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 20227-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 20227-1-AP (ARF5-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HeLa-Zellen, humanes Hodengewebe, Maushirngewebe, Maus-Kolongewebe, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

20227-1-AP bindet in WB, IF, ELISA ARF5-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ADP-ribosylation factor 5
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: NM_001662
• Gene symbol: ARF5
• Gene ID (NCBI): 381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). Their function are not fully described.The antibody is specific to ARF5.

Publikationen

Species	Application	Title
mouse	IF	PeerJ; Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection.; Authors - Ruichao Li
mouse	WB	Cell Rep; Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis; Authors - Rakhee Bajaj ; KD Validated