ARF5 Polyklonaler Antikörper

ARF5 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 15281-1-AP

Synonyme

ADP ribosylation factor 5, ARF5

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 15281-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15281-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 15281-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15281-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15281-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 15281-1-AP

  IP result of anti-ARF5 (IP:15281-1-AP, 4ug; Detection:15281-1-AP 1:500) with MCF-7 cells lysate 2840 ug.

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  IHC staining of human breast cancer using 15281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15281-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15281-1-AP (ARF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, humanes Lebergewebe, Maushirngewebe, PC-12-Zellen, Raji-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

15281-1-AP bindet in WB, IP, IHC, IF, ELISA ARF5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARF5 fusion protein Ag7400
• Vollständiger Name: ADP-ribosylation factor 5
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC003043
• Gene symbol: ARF5
• Gene ID (NCBI): 381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publikationen

Species	Application	Title
human	WB	J Physiol Biochem; Rab11-FIP4 interacts with ARF5 to promote cancer stemness in hepatocellular carcinoma; Authors - Feifeng Song ; KD Validated