ARF4 Monoklonaler Antikörper

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• KD/KO Validated

ARF4 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A4B8

Kat-Nr. : 68204-1-Ig

Synonyme

ADP ribosylation factor 4, ARF2, ARF4

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Galerie der Validierungsdaten

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  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 68204-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68204-1-Ig

  WB result of ARF4 antibody (68204-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

• 

  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68204-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 68204-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 68204-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARF4 antibody (68204-1-Ig, Clone: 2A4B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of K-562 using 68204-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (68204-1-Ig, Clone:2A4B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68204-1-Ig bindet in WB, IF, FC, ELISA ARF4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARF4 fusion protein Ag25522
• Vollständiger Name: ADP-ribosylation factor 4
• Berechnetes Molekulargewicht: 180 aa, 21 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC003364
• Gene symbol: ARF4
• Gene ID (NCBI): 378
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.