ARF1 Polyklonaler Antikörper

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• KD/KO Validated

ARF1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 20226-1-AP

Synonyme

ADP ribosylation factor 1, ARF1

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Galerie der Validierungsdaten

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  WB analysis using 20226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20226-1-AP

  WB result of ARF1 antibody (20226-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 20226-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20226-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20226-1-AP

  WB result of ARF1 antibody (20226-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HeLa cells.

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  WB analysis of HEK-293 using 20226-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20226-1-AP

  IP Result of anti-ARF1 (IP:20226-1-AP, 3ug; Detection:20226-1-AP 1:600) with HeLa cells lysate 2440ug.

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  IHC staining of human breast cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20226-1-AP (ARF1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20226-1-AP (ARF1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 20226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20226-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 20226-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARF1 (20226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, SKOV-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hirngewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

20226-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ARF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ADP-ribosylation factor 1
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 18-21 kDa
• GenBank-Zugangsnummer: NM_001658
• Gene symbol: ARF1
• Gene ID (NCBI): 375
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.

Publikationen

Species	Application	Title
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai
human,mouse	WB	Nat Commun; Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation; Authors - Mutian Jia
human	WB	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang
human	WB,IF	J Cell Sci; The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.; Authors - Yuanyuan Su
human	WB	Sci Rep; In vivo monitoring of the recruitment and activation of AP-1 by Arf1.; Authors - Etienne Sauvageau

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:20226-1-AP)