ARAF Polyklonaler Antikörper

ARAF Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 22129-1-AP

Synonyme

A RAF, ARAF, ARAF1, PKS, PKS2, Proto oncogene A Raf, Proto oncogene A Raf 1, Proto oncogene Pks, RAFA1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 22129-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22129-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22129-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 22129-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22129-1-AP (ARAF Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 22129-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22129-1-AP (ARAF Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

22129-1-AP bindet in WB, IHC, IF, ELISA ARAF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARAF fusion protein Ag17588
• Vollständiger Name: v-raf murine sarcoma 3611 viral oncogene homolog
• Berechnetes Molekulargewicht: 609 aa, 68 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC007514
• Gene symbol: ARAF
• Gene ID (NCBI): 369
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).

Publikationen

Species	Application	Title
human	IHC	Oncogene; Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.; Authors - Feifei Zhang
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse	WB,IF	Oncol Lett; Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.; Authors - Ying Xu
human	WB	Front Med; 5'-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I; Authors - Chengdong Wu