APPL1 Monoklonaler Antikörper

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• KD/KO Validated

APPL1 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B7B11

Kat-Nr. : 68195-1-Ig

Synonyme

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

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Galerie der Validierungsdaten

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  WB analysis using 68195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68195-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 68195-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68195-1-Ig

  WB result of APPL1 antibody (68195-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

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  IHC staining of human colon cancer using 68195-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68195-1-Ig (APPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68195-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (68195-1-Ig, Clone: 1B7B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 68195-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (68195-1-Ig, Clone:1B7B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Hausschwein-Herzgewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Kaninchenherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68195-1-Ig bindet in WB, IHC, IF, FC, ELISA APPL1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APPL1 fusion protein Ag3334
• Vollständiger Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• Berechnetes Molekulargewicht: 709 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC028599
• Gene symbol: APPL1
• Gene ID (NCBI): 26060
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].