APOBEC3G Monoklonaler Antikörper

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• KD/KO Validated

APOBEC3G Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

5G10A8

Kat-Nr. : 60100-1-Ig

Synonyme

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

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Galerie der Validierungsdaten

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  WB analysis of PC-3 cells using 60100-1-Ig

  WB result of APOBEC3G antibody (60100-1-Ig, 1:1000) with si-Control and si-APOBEC3G transfected PC-3 cells.

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  WB analysis of A375 using 60100-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60100-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 60100-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60100-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of C6 using 60100-1-Ig

  Immunofluorescent analysis of C6 cells using 60100-1-Ig (APOBEC3G antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

60100-1-Ig bindet in WB, IHC, IF APOBEC3G und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APOBEC3G fusion protein Ag7770
• Vollständiger Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC024268
• Gene symbol: APOBEC3G
• Gene ID (NCBI): 60489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Sci Rep; Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.; Authors - Ruidong Chen
human	IHC	Int J Clin Exp Med; APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.; Authors - Huanrong Lan