APOBEC3B Polyklonaler Antikörper

APOBEC3B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14559-1-AP

Synonyme

A3B, APOBEC1L, APOBEC3B, ARCD3, ARP4, bK150C2.2, Phorbolin 1 related protein, Phorbolin 2/3, PHRBNL

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Galerie der Validierungsdaten

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  WB analysis using 14559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14559-1-AP (APOBEC3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14559-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14559-1-AP (APOBEC3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14559-1-AP (APOBEC3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14559-1-AP (APOBEC3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14559-1-AP bindet in WB, IP, IHC, ELISA APOBEC3B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOBEC3B fusion protein Ag6115
• Vollständiger Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC053859
• Gene symbol: APOBEC3B
• Gene ID (NCBI): 9582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA cytosine deaminase APOBEC3B was identified recently as a source of DNA damage and mutagenesis in breast, head/neck, cervix, bladder, lung, ovary, and to lesser extents additional cancer types. This enzyme is also an effector protein in the innate immune response to virus infection. APOBEC3B has 3 isoforms produced by alternative splicing with the MW of 46, 29, 43 kDa. It can also exist as a dimer and can interact with APOBEC3G.

Publikationen

Species	Application	Title
human	IHC	J Med Genet; Multiregion whole-genome sequencing depicts intratumour heterogeneity and punctuated evolution in ovarian clear cell carcinoma.; Authors - Xia Yin
human	IHC	Front Oncol; Correlation Between APOBEC3B Expression and Clinical Characterization in Lower-Grade Gliomas.; Authors - Hao Zhang
human	IHC, WB, IP	Mol Carcinog; HPV-16/18 E6-induced APOBEC3B expression associates with proliferation of cervical cancer cells and hypomethylation of Cyclin D1.; Authors - Qiong Fan
human,mouse	WB,IHC	Zhongguo Fei Ai Za Zhi; [SWI/SNF Complex Gene Mutations Promote the Liver Metastasis of Non-small Cell Lung Cancer Cells in NSI Mice]; Authors - Lingling Gao