AP3S2 Polyklonaler Antikörper

AP3S2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC

Konjugation

Unkonjugiert

Kat-Nr. : 15319-1-AP

Synonyme

AP 3 complex subunit sigma 2, AP 3 complex subunit sigma 3B, AP3S2, AP3S3, Sigma 3B adaptin, Sigma adaptin 3b, sigma3b, Sigma3B adaptin

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Galerie der Validierungsdaten

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  WB analysis using 15319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15319-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 15319-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Hodengewebe, Maus-Eierstockgewebe, Maushodengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15319-1-AP bindet in WB, IHC AP3S2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP3S2 fusion protein Ag7565
• Vollständiger Name: adaptor-related protein complex 3, sigma 2 subunit
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC002785
• Gene symbol: AP3S2
• Gene ID (NCBI): 10239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP3S2 is a 22-kDa protein that belongs to the adaptor complexes small subunit family. AP3S2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-3 complex is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2).