ANKZF1 Polyklonaler Antikörper

ANKZF1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20447-1-AP

Synonyme

ANKZF1, Zinc finger protein 744, ZNF744

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 20447-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20447-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20447-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20447-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20447-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20447-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20447-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 20447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 20447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

20447-1-AP bindet in WB, IHC, IF, ELISA ANKZF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKZF1 fusion protein Ag14285
• Vollständiger Name: ankyrin repeat and zinc finger domain containing 1
• Berechnetes Molekulargewicht: 726 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC000238
• Gene symbol: ANKZF1
• Gene ID (NCBI): 55139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	bioRxiv; Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats; Authors - Yi-Ju Tseng