ANKRD27 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ANKRD27 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 24034-1-AP

Synonyme

ANKRD27, PP12899, VARP, VPS9 domain containing protein

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 24034-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24034-1-AP (ANKRD27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 24034-1-AP

  IP Result of anti-ANKRD27 (IP:24034-1-AP, 4ug; Detection:24034-1-AP 1:1000) with A431 cells lysate 2000ug.

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  IP experiment of MCF-7 using 24034-1-AP

  IP Result of anti-ANKRD27 (IP:24034-1-AP, 4ug; Detection:24034-1-AP 1:500) with MCF-7 cells lysate 3200ug.

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  IHC staining of human breast cancer using 24034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24034-1-AP (ANKRD27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 24034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24034-1-AP (ANKRD27 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 24034-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24034-1-AP (ANKRD27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche IP: A431-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

24034-1-AP bindet in WB, IP, IHC, IF, ELISA ANKRD27 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKRD27 fusion protein Ag21239
• Vollständiger Name: ankyrin repeat domain 27 (VPS9 domain)
• Berechnetes Molekulargewicht: 1050 aa, 117 kDa
• Beobachtetes Molekulargewicht: 117-130 kDa
• GenBank-Zugangsnummer: BC050529
• Gene symbol: ANKRD27
• Gene ID (NCBI): 84079
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VARP (VPS9-ankyrin-repeat protein, also known as ANKRD27) was originally identified as an N-terminal VPS9 (vacuolar protein sorting 9)-domain-containing protein that possesses guanine nucleotide exchange factor (GEF) activity toward small GTPase Rab21 and contains two ankyrin repeat (ANKR) domains in its central region.

Publikationen

Species	Application	Title
human	WB	EMBO J; Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy.; Authors - Ana Jimenez-Orgaz ; KO Validated
	WB	J Cell Sci; TI-VAMP/VAMP7-SNARE-Rab-GTPase interaction network within a ciliary membrane targeting complex.; Authors - Vasundhara Kandachar
human	WB	Autophagy; RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1; Authors - Yifei Pei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Francisco (Verified Customer) (04-12-2022)

We have tested the ANKRD27 antibody in Neuro2a, MelJuSo and HeLa cells. While the antibody works in the human cell lines tested, we could not see the same band in the Neuro2a lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:600

ANKRD27 Antibody Western Blot validation (1:600 dilution) in (Cat no:24034-1-AP)