ANGPTL4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ANGPTL4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 18374-1-AP

Synonyme

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, NL2, PGAR, pp1158, PSEC0166, UNQ171/PRO197

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Galerie der Validierungsdaten

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  WB analysis using 18374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18374-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18374-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18374-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18374-1-AP

  IP Result of anti-ANGPTL4 (IP:18374-1-AP, 4ug; Detection:18374-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human ovary using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

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  IF Staining of NIH/3T3 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen, Mausnierengewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Herzgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

18374-1-AP bindet in WB, IP, IHC, IF, ELISA ANGPTL4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANGPTL4 fusion protein Ag13237
• Vollständiger Name: angiopoietin-like 4
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC023647
• Gene symbol: ANGPTL4
• Gene ID (NCBI): 51129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Publikationen

Species	Application	Title
human	IP	J Clin Invest; Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.; Authors - Akrit Sodhi
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang
mouse	IF	J Pathol; ANGPTL4-αvβ3 integrin interaction counteracts hypoxia-induced vascular permeability by modulating Src signalling downstream of VEGFR2.; Authors - Elisa Gomez Perdiguero
human	IHC	Breast Cancer Res; An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer.; Authors - Misako Sato
human	IHC,WB	J Cell Mol Med; Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure.; Authors - Mingyang Li
human	WB,IHC	J Cell Mol Med; The long non-coding RNA PVT1 represses ANGPTL4 transcription through binding with EZH2 in trophoblast cell.; Authors - Yetao Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Niklas (Verified Customer) (11-28-2022)

bright and specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:350
• Cell Tissue Type: human fibroblasts