AMPK Alpha 1 Monoklonaler Antikörper

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• KD/KO Validated

AMPK Alpha 1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

60

CloneNo.

1H8E1

Kat-Nr. : 66536-1-Ig

Synonyme

AMPK, AMPK alpha, AMPK Alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66536-1-Ig

  WB result of AMPK alpha antibody (66536-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha transfected HEK-293 cells.

• 

  WB analysis using 66536-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66536-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66536-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66536-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66536-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66536-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, Jurkat-Zellen, K-562-Zellen, L02-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 58 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Produktinformation

66536-1-Ig bindet in WB, IHC, FC, ELISA AMPK Alpha 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte, Ziege
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AMPK Alpha 1 fusion protein Ag24744
• Vollständiger Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC012622
• Gene symbol: PRKAA1
• Gene ID (NCBI): 5562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.; Authors - Shenghao Zhang
human,hamster	WB	Biomed Pharmacother; Berberine is a potential alternative for metformin with good regulatory effect on lipids in treating metabolic diseases; Authors - Hui-Hui Guo
mouse	WB,IHC	J Nanobiotechnology; Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.; Authors - Chunai Gong
human	WB	J Nanobiotechnology; Graphene oxide exacerbates dextran sodium sulfate-induced colitis via ROS/AMPK/p53 signaling to mediate apoptosis.; Authors - Siliang Liu
human	WB,FC	Food Chem; Selective transportation and energy homeostasis regulation of dietary advanced glycation end-products in human intestinal Caco-2 cells.; Authors - Yi Wu
human	WB	Int J Mol Sci; Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.; Authors - Weiyun Zhang