AMOTL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AMOTL2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (2)

Kat-Nr. : 23351-1-AP

Synonyme

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 23351-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23351-1-AP (AMOTL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

23351-1-AP bindet in WB, IF, CoIP, ELISA AMOTL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMOTL2 fusion protein Ag19953
• Vollständiger Name: angiomotin like 2
• Berechnetes Molekulargewicht: 779 aa, 86 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC011454
• Gene symbol: AMOTL2
• Gene ID (NCBI): 51421
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta.(PMID: 34036399,PMID: 25080976)

Publikationen

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO.; Authors - Xue Zhang
human	WB	Mol Ther Nucleic Acids; Hypoxic Tumor-Derived Exosomal Long Noncoding RNA UCA1 Promotes Angiogenesis via miR-96-5p/AMOTL2 in Pancreatic Cancer.; Authors - Zengya Guo ; KD Validated
human	WB	Arthritis Res Ther; Regulation of type I interferon signature by VGLL3 in the fibroblast-like synoviocytes of rheumatoid arthritis patients via targeting the Hippo pathway; Authors - Yu Du
human	WB	Sci Rep; MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.; Authors - Diala Kantar ; KD Validated
human	WB	Mol Biol Cell; AMOT130 drives BMP-SMAD signaling at the apical membrane in polarized cells.; Authors - Patrizia Brunner ; KD Validated
human	WB	Pharmaceuticals (Basel); Comparative Assessment and High-Throughput Drug-Combination Profiling of TEAD-Palmitoylation Inhibitors in Hippo Pathway Deficient Mesothelioma; Authors - Lale Evsen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Florian (Verified Customer) (10-13-2021)

10% SDS-PAGE 1st AB incubation ON

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human breast cancer cell line (HCC1806)

AMOTL2 Antibody Western Blot validation (1:500 dilution) in Human breast cancer cell line (HCC1806) (Cat no:23351-1-AP)

FH

Lisa (Verified Customer) (07-08-2020)

Two specific bands (60 and 100 kDa) and two others non specific.This antibody is working by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1/600
• Cell Tissue Type: MCF7 cell line

AMOTL2 Antibody Western Blot validation (1/600 dilution) in MCF7 cell line (Cat no:23351-1-AP)