AMIGO3 Polyklonaler Antikörper

AMIGO3 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21278-1-AP

Synonyme

ALI3, Alivin 3, AMIGO 3, AMIGO3, Amphoterin induced protein 3, KIAA1851

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 21278-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21278-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21278-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21278-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21278-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 21278-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 21278-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21278-1-AP (AMIGO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HeLa-Zellen, humanes Hirngewebe, L02-Zellen, Maus-Eierstockgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21278-1-AP bindet in WB, IF,ELISA AMIGO3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMIGO3 fusion protein Ag15790
• Vollständiger Name: adhesion molecule with Ig-like domain 3
• Berechnetes Molekulargewicht: 504 aa, 55 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC110418
• Gene symbol: AMIGO3
• Gene ID (NCBI): 386724
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.