JAML Polyklonaler Antikörper

JAML Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 21302-1-AP

Synonyme

AMICA, AMICA1, CREA7 1, CREA7 4, Gm638, JAML

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Galerie der Validierungsdaten

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  WB analysis using 21302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21302-1-AP (JAML antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 21302-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 21302-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 21302-1-AP

  Immunofluorescent analysis of Hela cells, using AMICA1 antibody 21302-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 21302-1-AP

  1X10^6 HeLa cells were stained with 0.2ug JAML antibody (21302-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

21302-1-AP bindet in WB, IHC, IF, FC, ELISA JAML und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JAML fusion protein Ag15900
• Vollständiger Name: adhesion molecule, interacts with CXADR antigen 1
• Berechnetes Molekulargewicht: 394 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa, 50-70 kDa
• GenBank-Zugangsnummer: BC100797
• Gene symbol: AMICA1
• Gene ID (NCBI): 120425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Junctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062).

Publikationen

Species	Application	Title
human	IHC,IF	J Transl Med; Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma.; Authors - Qian Wu
mouse	WB,IHC	Eur J Pharmacol; Resveratrol ameliorates diabetic kidney injury by reducing lipotoxicity and modulates expression of components of the junctional adhesion molecule-like/sirtuin 1 lipid metabolism pathway.; Authors - Wei Gu
mouse	IHC,IF	Clin Sci (Lond); Silencing of junctional adhesion molecule-like protein attenuates atherogenesis and enhances plaque stability in ApoE-/- mice.; Authors - Yu Sun