AMH Polyklonaler Antikörper

AMH Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14461-1-AP

Synonyme

AMH, anti Mullerian hormone, MIF, MIS

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Galerie der Validierungsdaten

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  WB analysis of human testis using 14461-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14461-1-AP (AMH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Hodengewebe, humanes Ovarialkarzinomgewebe, humanes Pankreasgewebe, Ratten-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Eierstockgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

14461-1-AP bindet in WB, IHC, IF, ELISA AMH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Huhn, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMH fusion protein Ag5670
• Vollständiger Name: anti-Mullerian hormone
• Berechnetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC049194
• Gene symbol: AMH
• Gene ID (NCBI): 268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa. This antibody is specific to AMH.

Publikationen

Species	Application	Title
chicken	WB	Theriogenology; miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens; Authors - Bei-Bei Zhang ; KD Validated
mouse	WB	Exp Cell Res; Proteomic analysis of mouse ovaries during the prepubertal stages.; Authors - Jiaqiang Xiong
		Hum Reprod; Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis.; Authors - Hisashi Kataoka
mouse	IHC	J Funct Biomater; Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice; Authors - Tong Wu
rat	WB	Basic Clin Pharmacol Toxicol; Effects of quercetin on ovarian function and regulation of the ovarian PI3K/Akt/FoxO3a signalling pathway and oxidative stress in a rat model of cyclophosphamide-induced premature ovarian failure; Authors - Shaoyan Zheng
goat	WB	Theriogenology; BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells; Authors - Lei Wang