AMFR/GP78 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AMFR/GP78 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 16675-1-AP

Synonyme

AMF receptor, isoform 2, AMFR, GP78, RING finger protein 45, RNF45

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Galerie der Validierungsdaten

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  WB analysis using 16675-1-AP

  HepG2 lysates were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:4000 incubated at 37℃ and 100℃ for 1.5 hours.

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  WB analysis of HepG2 using 16675-1-AP

  WB result of AMFR antibody (16675-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMFR transfected HepG2 cells.

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  WB analysis of HepG2 using 16675-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16675-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 16675-1-AP

  IP result of anti-AMFR (IP:16675-1-AP, 4ug; Detection:16675-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

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  IHC staining of mouse ovary using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16675-1-AP (AMFR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16675-1-AP (AMFR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16675-1-AP

  Immunofluorescent analysis of HepG2 cells, using AMFR antibody 16675-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 16675-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human AMFR/GP78 (16675-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, bei 37°C inkubierte HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16675-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA AMFR/GP78 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMFR/GP78 fusion protein Ag10178
• Vollständiger Name: autocrine motility factor receptor
• Berechnetes Molekulargewicht: 643 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC069197
• Gene symbol: AMFR
• Gene ID (NCBI): 267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).

Publikationen

Species	Application	Title
mouse	WB	Cell; BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.; Authors - Fabien Llambi ; KD Validated
human, mouse	WB, IF	Immunity; The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.; Authors - Qiang Wang ; KD Validated
human	IF	PLoS Biol; Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.; Authors - Guang Gao
human	WB	EMBO Rep; MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.; Authors - Sandra Stefanovic-Barrett ; KO Validated
human	WB	Elife; Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.; Authors - Jianneng Li ; KD Validated
human	WB	Elife; The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.; Authors - Sam A Menzies