PLAP Polyklonaler Antikörper

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• KD/KO Validated

PLAP Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 18507-1-AP

Synonyme

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 18507-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18507-1-AP (PLAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18507-1-AP

  WB result of PLAP antibody (18507-1-AP, 1:500) with si-control and si-PLAP transfected HepG2 cells.

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  WB analysis of mouse liver using 18507-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18507-1-AP (PLAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18507-1-AP

  IP Result of anti-PLAP (IP:18507-1-AP, 4ug; Detection:18507-1-AP 1:700) with HepG2 cells lysate 1800ug.

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 18507-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PLAP (18507-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

18507-1-AP bindet in WB, IP, IHC, FC, ELISA PLAP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLAP fusion protein Ag13361
• Vollständiger Name: alkaline phosphatase, placental (Regan isozyme)
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC009647
• Gene symbol: ALPP
• Gene ID (NCBI): 250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

Publikationen

Species	Application	Title
mouse	WB	Aging (Albany NY); Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.; Authors - Jie Xu
human	IHC	ACS Biomater Sci Eng; 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model.; Authors - Song Shen
mouse	WB	Mol Cell Endocrinol; Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis.; Authors - Jinhui Wu
	WB	J Dent; PMID: 32119967; Authors - Yunxia Zhang
human	WB	Amino Acids; New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H2S-donor.; Authors - Halina Jurkowska
mouse	WB	Biomaterials; NRF2 is a critical regulator and therapeutic target of metal implant particle-incurred bone damage; Authors - Jian Dong

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C