ALG13 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ALG13 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 20810-1-AP

Synonyme

ALG13, CXorf45, GLT28D1, MDS031, YGL047W

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 20810-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20810-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20810-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 20810-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 20810-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

20810-1-AP bindet in WB, IHC, IF, ELISA ALG13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALG13 fusion protein Ag14763
• Vollständiger Name: asparagine-linked glycosylation 13 homolog
• Berechnetes Molekulargewicht: 165 aa, 18 kDa
• Beobachtetes Molekulargewicht: 130-135 kDa
• GenBank-Zugangsnummer: BC005336
• Gene symbol: ALG13
• Gene ID (NCBI): 79868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Inherit Metab Dis; Predominant and novel de novo variants in 29 individuals with ALG13 deficiency: Clinical description, biomarker status, biochemical analysis, and treatment suggestions.; Authors - Bobby G Ng
mouse	IF	Nephron; Dysregulation of the Expression of Asparagine-Linked Glycosylation 13 Short Isoform 2 Affects Nephrin Function by Altering Its N-Linked Glycosylation.; Authors - Teresa Esposito
mouse	WB,IHC,IF	Neuroscience; ALG13 Deficiency Associated with Increased Seizure Susceptibility and Severity.; Authors - Peng Gao ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kathryn (Verified Customer) (07-09-2020)

Works beautifully on cryopreserved ovarian tissue. Image provided is postnatal day 4 ovary stained with anti-ALG13 in red and anti-TRA98 in green.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: neonatal ovary

ALG13 Antibody Immunofluorescence, validation (1:100 dilution) in neonatal ovary (Cat no:20810-1-AP)