ALDOB Polyklonaler Antikörper

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• KD/KO Validated

ALDOB Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

30

Kat-Nr. : 18065-1-AP

Synonyme

ALDB, ALDOB, Liver type aldolase

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Galerie der Validierungsdaten

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  WB analysis using 18065-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18065-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18065-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18065-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18065-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18065-1-AP

  IP result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:500) with mouse brain tissue lysate 2400 ug.

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  IP experiment of mouse kidney using 18065-1-AP

  IP Result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver cancer using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maushirngewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe, Raji-Zellen, Rattennierengewebe
• Erfolgreiche IP: Maushirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

18065-1-AP bindet in WB, IP, IHC, IF, ELISA ALDOB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDOB fusion protein Ag12695
• Vollständiger Name: aldolase B, fructose-bisphosphate
• Berechnetes Molekulargewicht: 316 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC029399
• Gene symbol: ALDOB
• Gene ID (NCBI): 229
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

Publikationen

Species	Application	Title
mouse	WB	Nature; Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.; Authors - Chen-Song Zhang ; KD Validated
human	IF	Cancer Cell; Proteogenomic characterization identifies clinically relevant subgroups of intrahepatic cholangiocarcinoma.; Authors - Liangqing Dong
human	WB	Nat Commun; O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.; Authors - Kibum Kim
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
human	WB,IP	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma