ALDH9A1 Monoklonaler Antikörper

ALDH9A1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C5C9

Kat-Nr. : 67372-1-Ig

Synonyme

ALDH4, ALDH7, ALDH9, ALDH9A1, E3, TMABADH

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 67372-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67372-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67372-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67372-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67372-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67372-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67372-1-Ig bindet in WB, IHC, ELISA ALDH9A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALDH9A1 fusion protein Ag24493
• Vollständiger Name: aldehyde dehydrogenase 9 family, member A1
• Berechnetes Molekulargewicht: 518 aa, 56 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC151140
• Gene symbol: ALDH9A1
• Gene ID (NCBI): 223
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.