ALDH6A1 Polyklonaler Antikörper

ALDH6A1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20452-1-AP

Synonyme

ALDH6A1, MMSADHA, MMSDH

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Galerie der Validierungsdaten

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  WB analysis using 20452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20452-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20452-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, HEK-293-Zellen, Mausherzgewebe, Mauslebergewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:12000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

20452-1-AP bindet in WB, IHC, ELISA ALDH6A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH6A1 fusion protein Ag13879
• Vollständiger Name: aldehyde dehydrogenase 6 family, member A1
• Berechnetes Molekulargewicht: 535 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC004909
• Gene symbol: ALDH6A1
• Gene ID (NCBI): 4329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes.; Authors - Sven Wach
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
human	WB	Theranostics; DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting β-alanine and carnosine homeostasis; Authors - Jianan Zhao