ALDH3A2 Polyklonaler Antikörper

ALDH3A2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 15090-1-AP

Synonyme

Aldehyde dehydrogenase 10, ALDH10, ALDH3A2, FALDH, Fatty aldehyde dehydrogenase, SLS

---

Galerie der Validierungsdaten

• 

  WB analysis using 15090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15090-1-AP

  IP Result of anti-ALDH3A2 (IP:15090-1-AP, 4ug; Detection:15090-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

• 

  IP experiment of HEK-293 using 15090-1-AP

  IP result of anti-ALDH3A2(IP:15090-1-AP, 4ug; Detection:15090-1-AP 1:6000) with HEK-293 cells lysate 680 ug.

• 

  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15090-1-AP

  Immunofluorescent analysis of Hela cells, using ALDH3A2 antibody 15090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 15090-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ALDH3A2 antibody (15090-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

15090-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ALDH3A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH3A2 fusion protein Ag1687
• Vollständiger Name: aldehyde dehydrogenase 3 family, member A2
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: BC002430
• Gene symbol: ALDH3A2
• Gene ID (NCBI): 224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IHC	J Cell Mol Med; Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis.; Authors - Wei Chen
	IHC	Cells; Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models.; Authors - Marie-Anaïs Locquet
mouse	WB	J Biol Chem; Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.; Authors - Pamela A Young
		Front Med (Lausanne); Synthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein histone H3.1; Authors - Kristian Nzogang Fomo
human	WB	Am J Physiol Gastrointest Liver Physiol; Effect of an obesogenic high-fat and high-sucrose diet on hepatic gene expression signatures in male Collaborative Cross mice; Authors - Volodymyr P Tryndyak ; KD Validated
human	WB	Transl Oncol; miR-1182-mediated ALDH3A2 inhibition affects lipid metabolism and progression in ccRCC by activating the PI3K-AKT pathway; Authors - Qingyang Lv ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Elisabeth (Verified Customer) (09-02-2019)

HeLa cells treated with control (Lane 1) or ALDH3A2 (Lane 2) siRNA for 72h.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

ALDH3A2 Antibody Western Blot, validation (1:1000 dilution) in HeLa (Cat no:15090-1-AP)