ALDH1L1 Polyklonaler Antikörper

ALDH1L1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 17390-1-AP

Synonyme

10 FTHFDH, ALDH1L1, FTHFD

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Galerie der Validierungsdaten

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  WB analysis using 17390-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17390-1-AP (ALDH1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17390-1-AP

  IP Result of anti-ALDH1L1 (IP:17390-1-AP, 4ug; Detection:17390-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IP experiment of mouse liver using 17390-1-AP

  IP Result of anti-ALDH1L1 (IP:17390-1-AP, 4ug; Detection:17390-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 17390-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ALDH1L1 antibody (17390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, mouse liver, rat liver
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

17390-1-AP bindet in WB, IP, IHC, IF, ELISA ALDH1L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1L1 fusion protein Ag11285
• Vollständiger Name: aldehyde dehydrogenase 1 family, member L1
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC027241
• Gene symbol: ALDH1L1
• Gene ID (NCBI): 10840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

Publikationen

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
mouse	IHC,WB	Nat Commun; Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.; Authors - Ming Zhang
human	IHC,WB	NPJ Parkinsons Dis; Reduced astrocytic reactivity in human brains and midbrain organoids with PRKN mutations.; Authors - Masayoshi Kano
mouse	WB,IF	Gene; Immortalization of mouse primary astrocytes; Authors - Weihong Yi
human	IF	Curr Protoc; Primary Astrocytes Purification and Immortalization; Authors - Lei Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Juan (Verified Customer) (01-11-2022)

Working fine

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: hiPSC-derived astrocytes

ALDH1L1 Antibody Immunofluorescence validation (1:50 dilution) in hiPSC-derived astrocytes (Cat no:17390-1-AP)