ALDH1B1 Polyklonaler Antikörper

ALDH1B1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 22220-1-AP

Synonyme

Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 22220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22220-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22220-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22220-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22220-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 22220-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ALDH1B1 antibody 22220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Maushodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

22220-1-AP bindet in WB, IHC, IF, ELISA ALDH1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1B1 fusion protein Ag17557
• Vollständiger Name: aldehyde dehydrogenase 1 family, member B1
• Berechnetes Molekulargewicht: 517 aa, 57 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa
• GenBank-Zugangsnummer: BC001619
• Gene symbol: ALDH1B1
• Gene ID (NCBI): 219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

Publikationen

Species	Application	Title
rat	WB	Oncotarget; Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.; Authors - Hao-Long Zeng
human	IHC	Transl Cancer Res; ALDH1B1 predicts poor survival for locally advanced nasopharyngeal carcinoma patients.; Authors - Tao Zhu