ALDH1B1 Polyklonaler Antikörper

ALDH1B1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 15560-1-AP

Synonyme

Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15560-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15560-1-AP

  IP Result of anti-ALDH1B1 (IP:15560-1-AP, 4ug; Detection:15560-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15560-1-AP

  Immunofluorescent analysis of HepG2 cells using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below

Produktinformation

15560-1-AP bindet in WB, IP, IHC, IF, ELISA ALDH1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1B1 fusion protein Ag7144
• Vollständiger Name: aldehyde dehydrogenase 1 family, member B1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC001619
• Gene symbol: ALDH1B1
• Gene ID (NCBI): 219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
mouse	WB,IHC	J Pathol; Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice.; Authors - Mike F Müller
mouse	WB	Biomedicines; β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Am J Physiol Regul Integr Comp Physiol; Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.; Authors - Yuka Wakabayashi
mouse	WB	Am J Physiol Regul Integr Comp Physiol; Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice.; Authors - Akane Kasai