ALDH1A3 Polyklonaler Antikörper

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• KD/KO Validated

ALDH1A3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 25167-1-AP

Synonyme

Aldehyde dehydrogenase 6, ALDH1A3, ALDH1A6, ALDH6, RALDH 3, RALDH3, Retinaldehyde dehydrogenase 3

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Galerie der Validierungsdaten

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  WB analysis using 25167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25167-1-AP (ALDH1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 25167-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25167-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 25167-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25167-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25167-1-AP

  Immunofluorescent analysis of HepG2 cells using 25167-1-AP (ALDH1A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

25167-1-AP bindet in WB, IHC, IF, ELISA ALDH1A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1A3 fusion protein Ag18605
• Vollständiger Name: aldehyde dehydrogenase 1 family, member A3
• Berechnetes Molekulargewicht: 512 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC069274
• Gene symbol: ALDH1A3
• Gene ID (NCBI): 220
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), is an aldehyde dehydrogenase enzyme that uses retinal as a substrate either in a free or a cellular retinol-binding protein form. ALDH1A3 plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.ALDH1A3 is calculated to be 56 kDa.

Publikationen

Species	Application	Title
human	IF	Nature; A human embryonic limb cell atlas resolved in space and time; Authors - Bao Zhang
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
human	WB	Cell Res; Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.; Authors - Xingxing Xu
human	WB	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor
mouse	WB	Mol Metab; Autotaxin signaling facilitates β cell dedifferentiation and dysfunction induced by Sirtuin 3 deficiency.; Authors - Huanyi Cao
human	WB	Free Radic Biol Med; Flavonoid 4,4'-dimethoxychalcone suppresses cell proliferation via dehydrogenase inhibition and oxidative stress aggravation.; Authors - Changmei Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed mice spinal cord tissue by ALDH1A3 antibody at dilution of 1:100.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mice lumbar spinal cord tissue (transverse section 10 µm)