ALDH1A1-specific Polyklonaler Antikörper

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• KD/KO Validated

ALDH1A1-specific Polyklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 22109-1-AP

Synonyme

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

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Galerie der Validierungsdaten

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  WB analysis of human liver using 22109-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 22109-1-AP

  WB result of ALDH1A1 antibody (22109-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells.

• 

  WB analysis of human adrenal gland using 22109-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22109-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22109-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22109-1-AP (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22109-1-AP (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 22109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 22109-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1-specific (22109-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, humanes Nebennierengewebe, K-562-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

22109-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ALDH1A1-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1A1-specific fusion protein Ag17358
• Vollständiger Name: aldehyde dehydrogenase 1 family, member A1
• Berechnetes Molekulargewicht: 501 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC001505
• Gene symbol: ALDH1A1
• Gene ID (NCBI): 216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family. This antibody is specific to ALDH1A1.

Publikationen

Species	Application	Title
human,mouse	WB	Cancer Discov; Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.; Authors - Zhaolin Yang
human	WB	Theranostics; PMID: 28839463; Authors - Junlong Zhuang
human	IP	Commun Biol; PRMT3 interacts with ALDH1A1 and regulates gene-expression by inhibiting retinoic acid signaling.; Authors - Mamta Verma
human	WB	Int J Oncol; Parthenolide inhibits the tumor characteristics of renal cell carcinoma.; Authors - Dandan Liu
human	WB	Neoplasma; Long noncoding RNA ASAP1-IT1 promotes cancer stemness and predicts a poor prognosis in patients with bladder cancer.; Authors - L Yang
human	WB	Oncol Lett; Renal cell carcinoma with synchronous ipsilateral urothelial carcinoma of the renal pelvis.; Authors - Qun Lu