AKT3 Polyklonaler Antikörper

AKT3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 21641-1-AP

Synonyme

AKT3, PKB GAMMA, PKBG, PRKBG, Protein kinase Akt 3, Protein kinase B gamma, RAC gamma, RAC PK gamma, STK 2

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Galerie der Validierungsdaten

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  WB analysis using 21641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21641-1-AP (AKT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21641-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21641-1-AP (AKT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21641-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21641-1-AP (AKT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below

Produktinformation

21641-1-AP bindet in WB, IHC, ELISA AKT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKT3 fusion protein Ag16298
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
• Berechnetes Molekulargewicht: 465 aa, 54 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC121154
• Gene symbol: AKT3
• Gene ID (NCBI): 10000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT3, also named as PKBG, is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.; Authors - Yi-Yuan Sun
human	WB	J Cell Mol Med; EGR1 interacts with DNMT3L to inhibit the transcription of miR-195 and plays an anti-apoptotic role in the development of gastric cancer.; Authors - Yang Yang
human	IHC	Clin Epigenetics; Alterations of DNA methylation were associated with the rapid growth of cortisol-producing adrenocortical adenoma during pregnancy.; Authors - Chuan Wang
human	WB	Cancer Cell Int; NEK7 promotes gastric cancer progression as a cell proliferation regulator.; Authors - Yi-Ke Li
human	WB	Exp Hematol Oncol; YTHDF1 promotes hepatocellular carcinoma progression via activating PI3K/AKT/mTOR signaling pathway and inducing epithelial-mesenchymal transition.; Authors - Xiangyuan Luo
human	WB	Sci Rep; Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.; Authors - Jie Liu