AKT Polyklonaler Antikörper

AKT Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 55230-1-AP

Synonyme

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 55230-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55230-1-AP (AKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55230-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55230-1-AP (AKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

55230-1-AP bindet in WB, IHC, ELISA AKT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56-62 kDa
• GenBank-Zugangsnummer: NM_001014431
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT1, also named as PKB and RAC, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily. It plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. AKT1 promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. It plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. AKT1 mediates the antiapoptotic effects of IGF-I. (PMID:16139227)This antibody is specific to AKT1. It has no cross reaction to AKT2 and AKT3.

Publikationen

Species	Application	Title
human	WB	Mol Med Rep; Matrine inhibits the invasive and migratory properties of human hepatocellular carcinoma by regulating epithelial‑mesenchymal transition.; Authors - Yuwen Wang
mouse	WB	J Immunol; The Intracellular Interaction of Porcine β-Defensin 2 with VASH1 Alleviates Inflammation via Akt Signaling Pathway.; Authors - Lu Lu Li
mouse	WB	Int J Mol Sci; Extracellular Calcium-Induced Calcium Transient Regulating the Proliferation of Osteoblasts through Glycolysis Metabolism Pathways; Authors - Xiaohang Gao
rat	WB	J Ethnopharmacol; Shenqu xiaoshi oral solution enhances digestive function and stabilizes the gastrointestinal microbiota of juvenile rats with infantile anorexia; Authors - Meng Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution and 1:100 in IF

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Tanusree (Verified Customer) (08-14-2019)

The antibody works good in mouse brain tissue in western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain