AKR1B10 Polyklonaler Antikörper

AKR1B10 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 18252-1-AP

Synonyme

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HSI, SI reductase, Small intestine reductase

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Galerie der Validierungsdaten

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  WB analysis using 18252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18252-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18252-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HepG2-Zellen, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18252-1-AP bindet in WB, ELISA AKR1B10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKR1B10 fusion protein Ag13042
• Vollständiger Name: aldo-keto reductase family 1, member B10 (aldose reductase)
• Berechnetes Molekulargewicht: 316 aa, 36 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC008837
• Gene symbol: AKR1B10
• Gene ID (NCBI): 57016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we detected bands at different positions, but in cancer cell lines, the bands appeared notably clearer. The optimal concentration was determined to be higher, specifically at 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines and tumor tissues

AKR1B10 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines and tumor tissues (Cat no:18252-1-AP)