AKR1B1 Polyklonaler Antikörper

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• KD/KO Validated

AKR1B1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 15439-1-AP

Synonyme

ADR, AKR1B1, Aldehyde reductase, Aldose reductase, ALDR1, ALR2, AR

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Galerie der Validierungsdaten

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  WB analysis of A431 using 15439-1-AP

  WB result of AKR1B1 antibody (15439-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected A431 cells.

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  WB analysis using 15439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15439-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15439-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15439-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15439-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15439-1-AP (AKR1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, BxPC-3-Zellen, HepG2-Zellen, humanes Lebergewebe, humanes Plazenta-Gewebe, L02-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

15439-1-AP bindet in WB, IHC, IF, ELISA AKR1B1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKR1B1 fusion protein Ag7142
• Vollständiger Name: aldo-keto reductase family 1, member B1 (aldose reductase)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000260
• Gene symbol: AKR1B1
• Gene ID (NCBI): 231
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Oxid Med Cell Longev; WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.; Authors - Xiaoyu Zhou
human	WB	Biochem Pharmacol; Regulation on SIRT1-PGC-1α/Nrf2 pathway together with selective inhibition of aldose reductase makes compound hr5F a potential agent for the treatment of diabetic complications.; Authors - Zhihua Wang
rat	WB	Biomed Pharmacother; Systematic investigation of the pharmacological mechanism for renal protection by the leaves of Eucommia ulmoides Oliver using UPLC-Q-TOF/MS combined with network pharmacology analysis.; Authors - Qi Huang
rat	WB	Toxicol Appl Pharmacol; Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.; Authors - Zhanjun Ma
human	WB	BMC Nephrol; Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.; Authors - Yang Lu
human	WB	Curr Issues Mol Biol; AKR1B1 Represses Glioma Cell Proliferation through p38 MAPK-Mediated Bcl-2/BAX/Caspase-3 Apoptotic Signaling Pathways; Authors - Yu-Kai Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (01-12-2024)

Good antibody for studying hypertonic stress response.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: IMCD3 cells