AKR1A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AKR1A1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15054-1-AP

Synonyme

AKR1A1, Alcohol dehydrogenase [NADP+], Aldehyde reductase, ALDR1, ALR, ARM, DD3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15054-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15054-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15054-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15054-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15054-1-AP

  IP Result of anti-AKR1A1 (IP:15054-1-AP, 4ug; Detection:15054-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of HeLa using 15054-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15054-1-AP (AKR1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Lebergewebe, L02-Zellen, Mauslungengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

15054-1-AP bindet in WB, IP, IHC, IF, ELISA AKR1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKR1A1 fusion protein Ag7037
• Vollständiger Name: aldo-keto reductase family 1, member A1 (aldehyde reductase)
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000670
• Gene symbol: AKR1A1
• Gene ID (NCBI): 10327
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC,IP	Nature; Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.; Authors - Hua-Lin Zhou ; KO Validated
mouse	WB	J Med Chem; Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury; Authors - Hua-Lin Zhou
mouse	WB	J Biol Chem; AKR1A1 is a novel mammalian S-nitroso-glutathione reductase.; Authors - Colin T Stomberski ; KO Validated