AKAP12 Polyklonaler Antikörper

AKAP12 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (2)

Kat-Nr. : 25199-1-AP

Synonyme

A kinase anchor protein 12, AKAP 12, AKAP 250, AKAP12, AKAP250, Gravin, Myasthenia gravis autoantigen

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Galerie der Validierungsdaten

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  WB analysis using 25199-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 25199-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 25199-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

25199-1-AP bindet in WB, IHC, IF, ELISA AKAP12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP12 fusion protein Ag17769
• Vollständiger Name: A kinase (PRKA) anchor protein 12
• Berechnetes Molekulargewicht: 1782 aa, 191 kDa
• Beobachtetes Molekulargewicht: 200-300 kDa
• GenBank-Zugangsnummer: BC157067
• Gene symbol: AKAP12
• Gene ID (NCBI): 9590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)

Publikationen

Species	Application	Title
human	IHC	J Transl Med; Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy; Authors - Xiaohui Yang
human	WB	Mol Ther Nucleic Acids; The Human Novel Gene LNC-HC Inhibits Hepatocellular Carcinoma Cell Proliferation by Sequestering hsa-miR-183-5p.; Authors - Xi Lan
human	WB,IHC	Front Endocrinol (Lausanne); Construction of novel hypoxia-related gene model for prognosis and tumor microenvironment in endometrial carcinoma; Authors - Junfeng Chen
mouse	IF	Sci Rep; β-catenin regulates the formation of multiple nephron segments in the mouse kidney.; Authors - Patrick Deacon
mouse	IF	Surgery; Heat shock protein A12B gene therapy improves perfusion, promotes neovascularization, and decreases fibrosis in a murine model of hind limb ischemia.; Authors - Mahesh Thirunavukkarasu
human	WB	Am J Transl Res; Comprehensive analysis of differentially expressed non-coding RNAs and mRNAs in gastric cancer cells under hypoxic conditions.; Authors - Jia Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pradeep (Verified Customer) (07-20-2020)

I used for immunofluorescence in mouse tissue sample. it is working great.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse HLI

FH

Rushita (Verified Customer) (11-26-2019)

The Ab works very well for detection of endogenous AKAP12 for western blotting (1:1000) in HEK 293 and COS7 cell lines.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, COS7