AIM2 Monoklonaler Antikörper

AIM2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

3E12A8

Kat-Nr. : 66902-1-Ig

Synonyme

absent in melanoma 2, AIM2, PYHIN4

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Galerie der Validierungsdaten

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  WB analysis using 66902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit spleen using 66902-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66902-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66902-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 66902-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66902-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 66902-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, humanes Herzgewebe, Kaninchenmilzgewebe, Sp2/0-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

66902-1-Ig bindet in WB, IHC, IF, CoIP, ELISA AIM2 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AIM2 fusion protein Ag14607
• Vollständiger Name: absent in melanoma 2
• Berechnetes Molekulargewicht: 343 aa, 39 kDa
• Beobachtetes Molekulargewicht: 37-39 and 53 kDa
• GenBank-Zugangsnummer: BC010940
• Gene symbol: AIM2
• Gene ID (NCBI): 9447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

Publikationen

Species	Application	Title
human	CoIP	Gut; STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.; Authors - Ruby E Dawson
human	WB	Theranostics; Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy; Authors - Qingyu Liang
human	WB	Front Oncol; PKIB involved in the metastasis and survival of osteosarcoma; Authors - Rongxue Wan
mouse	WB	Cell Commun Signal; Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.; Authors - Dong-Ming Wu
mouse	WB	Sci Total Environ; Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway.; Authors - Jinzhao Zhou
mouse	WB,IF	EMBO J; Prohibitin 1 regulates mtDNA release and downstream inflammatory responses; Authors - Hao Liu ; KD Validated