AIDA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AIDA Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 23724-1-AP

Synonyme

AIDA, C1orf80, RP11 378J18.7

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 23724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23724-1-AP (AIDA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23724-1-AP

  IP Result of anti-AIDA (IP:23724-1-AP, 3ug; Detection:23724-1-AP 1:600) with HeLa cells lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 0x lens).

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  IHC staining of mouse skeletal muscle using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

23724-1-AP bindet in WB, IP, IHC, IF,ELISA AIDA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIDA fusion protein Ag20588
• Vollständiger Name: axin interactor, dorsalization associated
• Berechnetes Molekulargewicht: 306 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC015535
• Gene symbol: AIDA
• Gene ID (NCBI): 64853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

Publikationen

Species	Application	Title
mouse	IP	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi ; KO Validated
mouse	WB,IF	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (02-22-2022)

Unfortunately, the antibody-conjugated magnetic beads did not show a strong signal for the Luminex-based assay.

• Applications: Other
• Primary Antibody Dilution: 10ug/mL
• Cell Tissue Type: Human