AHR Monoklonaler Antikörper

AHR Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D1F9

Kat-Nr. : CL488-67785

Synonyme

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

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Galerie der Validierungsdaten

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  FC experiment of HepG2 using CL488-67785

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human AHR (CL488-67785, Clone:2D1F9) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67785 bindet in FC (Intra) AHR und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AHR fusion protein Ag28935
• Vollständiger Name: aryl hydrocarbon receptor
• Berechnetes Molekulargewicht: 848 aa, 96 kDa
• Beobachtetes Molekulargewicht: 105-110 kDa
• GenBank-Zugangsnummer: BC070080
• Gene symbol: AHR
• Gene ID (NCBI): 196
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C