AHR Polyklonaler Antikörper

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• KD/KO Validated

AHR Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 28727-1-AP

Synonyme

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

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Galerie der Validierungsdaten

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  WB analysis using 28727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28727-1-AP (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28727-1-AP (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 28727-1-AP

  WB result of AHR antibody (28727-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHR transfected HepG2 cells.

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  IP experiment of PC-3 using 28727-1-AP

  IP result of anti-AHR(IP:28727-1-AP, 4ug; Detection:28727-1-AP 1:5000) with PC-3 cells lysate 1480 ug.

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  IP experiment of HepG2 using 28727-1-AP

  IP result of anti-AHR(IP:28727-1-AP, 4ug; Detection:28727-1-AP 1:5000) with HepG2 cells lysate 1360 ug.

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  IHC staining of mouse small intestine using 28727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28727-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 28727-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28727-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28727-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AHR antibody (28727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HepG2-Zellen
• Erfolgreiche IP: PC-3-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

28727-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA AHR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AHR fusion protein Ag28935
• Vollständiger Name: aryl hydrocarbon receptor
• Berechnetes Molekulargewicht: 848 aa, 96 kDa
• Beobachtetes Molekulargewicht: 105-110 kDa
• GenBank-Zugangsnummer: BC070080
• Gene symbol: AHR
• Gene ID (NCBI): 196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Publikationen

Species	Application	Title
human	WB	Eur J Med Res; Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells; Authors - Chao Li
human	WB,IHC	Int Immunopharmacol; Paeoniflorin-6'-o-benzene sulfonate ameliorates the progression of adjuvant-induced arthritis by inhibiting the interaction between Ahr and GRK2 of fibroblast-like synoviocytes.; Authors - Bin-Jie Zhang
mouse	IHC	J Ethnopharmacol; Ginsenoside Rg1 protects mice against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced liver injury by inhibiting CYP1A1 through the aryl hydrocarbon receptor.; Authors - Han Li
mouse,human	WB	Toxicol Res (Camb); High-fat diet and alcohol induced-mice could cause colonic injury through molecular mechanisms of endogenous toxins; Authors - Shumin Zhu
mouse	WB,IF	Cell Prolif; Porphyromonas gingivalis infection promotes inflammation via inhibition of the AhR signalling pathway in periodontitis; Authors - Ting Hao
human	WB,IF	Oncol Lett; Fenofibrate attenuates the cytotoxic effect of cisplatin on lung cancer cells by enhancing the antioxidant defense system in vitro; Authors - Mariko Kogami