AHNAK Polyklonaler Antikörper

AHNAK Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16637-1-AP

Synonyme

AHNAK, AHNAK nucleoprotein, AHNAKRS, Desmoyokin, PM227

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Galerie der Validierungsdaten

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  WB analysis using 16637-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 16637-1-AP (AHNAK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHNAK antibody (16637-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

16637-1-AP bindet in WB, IHC, IF, FC, ELISA AHNAK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AHNAK fusion protein Ag9986
• Vollständiger Name: AHNAK nucleoprotein
• Berechnetes Molekulargewicht: 629 kDa
• GenBank-Zugangsnummer: BC000926
• Gene symbol: AHNAK
• Gene ID (NCBI): 79026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AHNAK, also known as desmoyokin, is described as a giant scaffold protein based on its large size (629 kDa) and ability to interact with different proteins to form multi-protein complexes. The bulk of the protein is assembled in 128-residue repetitive elements known as the central repeated unit (CRU). Its proposed functions are quite diverse, ranging from a role in the formation of the blood brain barrier, in cell architecture and migration, to the regulation of cardiac channels and muscle membrane repair. AHNAK is differentially expressed in some cancer cell lines. The expression of AHNAK is subsequently localized to the plasma membrane of keratinocytes in human epidermis. AHNAK has been reported in many intracellular locations including the nucleus, cytoplasm and plasma membrane.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.; Authors - Yuta Asano
human	IF	EMBO Rep; Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging; Authors - Monami Ogura
human	FC	Clin Exp Immunol; The AHNAK induces increased IL-6 production in CD4+T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss.; Authors - Liman Li