AHI1 Polyklonaler Antikörper

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• KD/KO Validated

AHI1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 22045-1-AP

Synonyme

AHI 1, AHI1, JBTS3, Jouberin, ORF1

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Galerie der Validierungsdaten

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  WB analysis using 22045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22045-1-AP (AHI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 5 publications below

Produktinformation

22045-1-AP bindet in WB, IF, ELISA AHI1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AHI1 fusion protein Ag16885
• Vollständiger Name: Abelson helper integration site 1
• Berechnetes Molekulargewicht: 1196 aa, 137 kDa
• Beobachtetes Molekulargewicht: 137-150 kDa
• GenBank-Zugangsnummer: BC094800
• Gene symbol: AHI1
• Gene ID (NCBI): 54806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AHI1, encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. AHI1 is expressed strongly in embryonic hindbrain and forebrain. AHI1 is involved in vesicle trafficking and is required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. AHI1, as a positive modulator of classical Wnt signaling, may play a key role in ciliary signaling during cerebellar embryonic development (PMID: 15467982, PMID: 21623382).

Publikationen

Species	Application	Title
human	IF	Cell Rep; A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.; Authors - Kari-Anne M Frikstad
human	IF	Front Cell Dev Biol; Superresolution Microscopy Reveals Distinct Phosphoinositide Subdomains Within the Cilia Transition Zone.; Authors - Sarah E Conduit
mouse	IF,WB	Mol Biol Cell; Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.; Authors - Yin Loon Lee ; KD Validated
human	IF	Sci Rep; Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.; Authors - Elisa Molinari
mouse	IF	Front Cell Dev Biol; EVC-EVC2 complex stability and ciliary targeting are regulated by modification with ubiquitin and SUMO; Authors - Pablo Barbeito