ADRA1A-Specific Polyklonaler Antikörper

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• KD/KO Validated

ADRA1A-Specific Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19777-1-AP

Synonyme

ADRA1A, ADRA1A-Specific, ADRA1C, ADRA1L1, Alpha 1A adrenergic receptor, Alpha 1A adrenoceptor, Alpha 1A adrenoreceptor, Alpha 1C adrenergic receptor, Alpha adrenergic receptor 1c, ALPHA1AAR

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Galerie der Validierungsdaten

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  WB analysis using 19777-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours without or with blocking peptide (1.4 ug peptide was used to block the binding of 1.0 ug antibody).

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  WB analysis of HeLa using 19777-1-AP

  WB result of ADRA1A-Specific antibody (19777-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRA1A-Specific transfected HeLa cells.

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  WB analysis using 19777-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 19777-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ADRA1A-Specific antibody (19777-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

19777-1-AP bindet in WB, IHC, IF, FC, ELISA ADRA1A-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: adrenergic, alpha-1A-, receptor
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_000680
• Gene symbol: ADRA1A
• Gene ID (NCBI): 148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.

Publikationen

Species	Application	Title
rat	WB	iScience; Changes in transcriptomic landscape in human end-stage heart failure with distinct etiology.; Authors - Miaomiao Zhu
human	IHC,IF	Pharmaceuticals (Basel); Vascular α1A Adrenergic Receptors as a Potential Therapeutic Target for IPAD in Alzheimer's Disease.; Authors - Miles Frost
human	WB	Vascul Pharmacol; The ERK1/2 pathway participates in the upregulation of the expression of mesenteric artery α1 receptors by intravenous tail injections of mmLDL in mice.; Authors - Jun-Jie Wang
mouse	WB	Heliyon; Luteolin improves vasoconstriction function and survival of septic mice via AMPK/NF-κB pathway; Authors - Bin Liu