ADHFE1 Polyklonaler Antikörper

ADHFE1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19359-1-AP

Synonyme

ADH8, ADHFE1, HMFT2263, HOT

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 19359-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19359-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19359-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 19359-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19359-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 19359-1-AP

  IP Result of anti-ADHFE1 (IP:19359-1-AP, 4ug; Detection:19359-1-AP 1:300) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human colon using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19359-1-AP bindet in WB, IP, IHC, ELISA ADHFE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADHFE1 fusion protein Ag6803
• Vollständiger Name: alcohol dehydrogenase, iron containing, 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC064634
• Gene symbol: ADHFE1
• Gene ID (NCBI): 137872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.