Gamma Adducin Polyklonaler Antikörper

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• KD/KO Validated

Gamma Adducin Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17585-1-AP

Synonyme

ADD3, ADDL, adducin 3 (gamma), Adducin like protein 70, Gamma adducin

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 17585-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17585-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 17585-1-AP

  IP Result of anti-Gamma adducin (IP:17585-1-AP, 4ug; Detection:17585-1-AP 1:500) with NIH/3T3 cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, K-562-Zellen, Mausnierengewebe
• Erfolgreiche IP: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

17585-1-AP bindet in WB, IP, ELISA Gamma Adducin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Gamma Adducin fusion protein Ag11318
• Vollständiger Name: adducin 3 (gamma)
• Berechnetes Molekulargewicht: 76 kDa, 79 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 90 kDa
• GenBank-Zugangsnummer: BC062559
• Gene symbol: ADD3
• Gene ID (NCBI): 120
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Mol Cell Biol; QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer.; Authors - Jin-Zhu Wang
human	WB	Stem Cell Res Ther; Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived stem cells via miR-143-3p/ADD3 axis; Authors - Hewei Xiong ; KD Validated
human	WB	PLoS One; Dysferlin and other non-red cell proteins accumulate in the red cell membrane of diamond-blackfan anemia patients.; Authors - Esther N Pesciotta
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda