ACSL3 Polyklonaler Antikörper

ACSL3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30214-1-AP

Synonyme

ACS3, ACSL3, FACL3, LACS 3, LACS3, PRO2194

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Galerie der Validierungsdaten

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  WB analysis using 30214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30214-1-AP (ACSL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30214-1-AP (ACSL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of LNCaP using 30214-1-AP

  IP result of anti-ACSL3(IP:30214-1-AP, 4ug; Detection:30214-1-AP 1:4000) with LNCaP cells lysate 1360 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HuH-7-Zellen, LNCaP-Zellen, Mausnierengewebe, MKN-45-Zellen
• Erfolgreiche IP: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30214-1-AP bindet in WB, IP, ELISA ACSL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSL3 fusion protein Ag33005
• Vollständiger Name: acyl-CoA synthetase long-chain family member 3
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC041692
• Gene symbol: ACSL3
• Gene ID (NCBI): 2181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.