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ACSL3 Polyklonaler Antikörper

ACSL3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20710-1-AP

Synonyme

ACS3, ACSL3, FACL3, LACS 3, LACS3, PRO2194



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HuH-7-Zellen, LNCaP-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 10 publications below
IHCSee 2 publications below

Produktinformation

20710-1-AP bindet in WB, IHC, ELISA ACSL3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name acyl-CoA synthetase long-chain family member 3
Berechnetes Molekulargewicht 80 kDa
Beobachtetes Molekulargewicht 70-80 kDa
GenBank-ZugangsnummerNM_004457
Gene symbol ACSL3
Gene ID (NCBI) 2181
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

Protokolle

Produktspezifische Protokolle
WB protocol for ACSL3 antibody 20710-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Commun (Lond)

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

Authors - Xiaoteng Cui
humanWB

Clin Transl Med

NAT10: An RNA cytidine transferase regulates fatty acid metabolism in cancer cells

Authors - Mahmood Hassan Dalhat
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu

Sci Rep

Morphologically and Functionally Distinct Lipid Droplet Subpopulations.

Authors - Shuyan Zhang
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Rab18 binds PLIN2 and ACSL3 to mediate lipid droplet dynamics.

Authors - Yaqin Deng
humanWB

Chem Biol Interact

Transcriptomic analysis suggested the involvement of impaired lipid droplet biogenesis in graphene oxide-induced cytotoxicity in human umbilical vein endothelial cells.

Authors - Yingmei Luo