ACRV1 Polyklonaler Antikörper

ACRV1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 14040-1-AP

Synonyme

Acrosomal protein SP 10, acrosomal vesicle protein 1, ACRV1, D11S4365, SP 10, SPACA2

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Galerie der Validierungsdaten

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  WB analysis of DU 145 using 14040-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14040-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 14040-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of DU 145 using 14040-1-AP

  IP Result of anti-ACRV1 (IP:14040-1-AP, 3ug; Detection:14040-1-AP 1:2000) with DU 145 cells lysate 560ug.

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  IHC staining of human testis using 14040-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14040-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-3 using 14040-1-AP

  Immunofluorescent analysis of PC-3 cells using 14040-1-AP (ACRV1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche IP: DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 10 publications below

Produktinformation

14040-1-AP bindet in WB, IP, IHC, IF, ELISA ACRV1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACRV1 fusion protein Ag5109
• Vollständiger Name: acrosomal vesicle protein 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 13-36 kDa
• GenBank-Zugangsnummer: BC014588
• Gene symbol: ACRV1
• Gene ID (NCBI): 56
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein ACRV1, also known as SP-10, is an acrosomal matrix protein, which is evolutionarily conserved among mammals. It is associated with the matrix of the acrosomal vesicle in developing spermatids and the acrosomal matrix and membranes of ejaculated sperm (PMID: 8288254). Human ACRV1 protein has 11 isoforms, and molecular weight ranges from 9 to 28 kDa. ACRV1 was predominantly located in round and elongated spermatids in the testis, indicating that ACRV1 may play an important role in mammalian spermatogenesis and may be a target of a contraceptive vaccine (PMID: 21488928; 8874713).

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.; Authors - Deqiang Ding
mouse	IHC	Int J Mol Sci; BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.; Authors - Chao Huang
rat	IF	Syst Biol Reprod Med; Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering; Authors - Vitória Frias Batista
mouse	IF	Bioinformatics; SATINN: an automated neural network-based classification of testicular sections allows for high-throughput histopathology of mouse mutants; Authors - Ran Yang
mouse	IF	Nat Commun; SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility; Authors - Jiali Liu
mouse	IF	Arch Toxicol; Extended exposure to tetrabromobisphenol A-bis(2,3-dibromopropyl ether) leads to subfertility in male mice at the late reproductive age; Authors - Yuan-Yuan Li