ACPP Polyklonaler Antikörper

ACPP Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15840-1-AP

Synonyme

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 15840-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15840-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15840-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15840-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15840-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-3 using 15840-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15840-1-AP (ACPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, COLO 320-Zellen, Maushodengewebe, Mauslungengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

15840-1-AP bindet in WB, IP, IHC, IF, ELISA ACPP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACPP fusion protein Ag8592
• Vollständiger Name: acid phosphatase, prostate
• Berechnetes Molekulargewicht: 418 aa, 48 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC007460
• Gene symbol: ACPP
• Gene ID (NCBI): 55
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Publikationen

Species	Application	Title
chicken	IHC	Biol Reprod; Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas.; Authors - Hyocheol Bae
mouse	IHC	Am J Pathol; PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer.; Authors - Wen-Cheng Chung
mouse	WB,IF,IP	Eur J Pain; Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice.; Authors - Xiaoxin Zhou
Rat	IHC, IF	Biochem Biophys Res Commun; Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats.; Authors - Whasun Lim