ACP2 Polyklonaler Antikörper

ACP2 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15236-1-AP

Synonyme

acid phosphatase 2, lysosomal, ACP2, LAP, Lysosomal acid phosphatase

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Galerie der Validierungsdaten

• 

  WB analysis of mouse kidney using 15236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15236-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15236-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15236-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15236-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15236-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15236-1-AP

  Immunofluorescent analysis of HepG2 cells using 15236-1-AP (ACP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HepG2-Zellen, HL-60-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15236-1-AP bindet in WB, IF,ELISA ACP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACP2 fusion protein Ag7356
• Vollständiger Name: acid phosphatase 2, lysosomal
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 63 kDa, 52 kDa
• GenBank-Zugangsnummer: BC003160
• Gene symbol: ACP2
• Gene ID (NCBI): 53
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.